Hotspot

trnK-UUU-rps16

LSC · 4354-5213 · 860 bp

Back to Cheirodendron

Pi 0.00116

MarkerSeek score 59.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 1
pi: 0.00116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCTTCGCTTCATCCA	GCCTACAGTAACTGTTCGGG	50.0	55.0	58.1	58.0	994-995 bp	1.0	79.6
trnK-UUU-rps16_p2	CCGATGGTATGGACGAATCC	GCCTACAGTAACTGTTCGGG	55.0	55.0	57.9	58.0	1007-1008 bp	1.0	79.4
trnK-UUU-rps16_p3	CGAATCCTTCGCTTCATCCA	CGCTCAGCCTACAGTAACTG	50.0	55.0	58.1	58.1	1000-1001 bp	1.0	78.9
trnK-UUU-rps16_p4	CCGATGGTATGGACGAATCC	CGCTCAGCCTACAGTAACTG	55.0	55.0	57.9	58.1	1013-1014 bp	1.0	78.8
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GCCTACAGTAACTGTTCGGG	50.0	55.0	58.2	58.0	945-946 bp	1.0	78.7