Hotspot

petD

LSC · 75207-76429 · 1223 bp

Back to Chasmanthium

Pi 0.00245

MarkerSeek score 60.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1223
variable_sites: 3
pi: 0.00245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACTTCTCTTCAACTCCGAAGT	GGACTTCGCGGTTGAATGAA	42.9	50.0	56.6	58.8	1332 bp	1.0	54.3
petD_p2	ACTTCTCTTCAACTCCGAAGT	GACTTCGCGGTTGAATGAATC	42.9	47.6	56.6	58.0	1331 bp	1.0	52.8
petD_p3	ACGAAAGAAAATAAGGCGGA	GGACTTCGCGGTTGAATGAA	40.0	50.0	55.4	58.8	1281 bp	1.0	52.4
petD_p4	ACTTCTCTTCAACTCCGAAGT	GGACTTCGCGGTTGAATGA	42.9	52.6	56.6	58.2	1332 bp	1.0	51.8
petD_p5	ACTTCTCTTCAACTCCGAAGT	CTTCGCGGTTGAATGAATCA	42.9	45.0	56.6	56.8	1329 bp	1.0	51.6