Hotspot

rps15-ndhF

IRb · 105428-105842 · 415 bp

Back to Chasmanthium

Pi 0

MarkerSeek score 33.60

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 415
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.34
markerseek_score: 33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	ATCCCGTGAAATTCTCGAGC	ATGGAGTAGGTCTTGCTGGT	50.0	50.0	58.1	58.0	849-1146 bp	1.0	83.4
rps15-ndhF_p2	ATTCTCGAGCCGAAAGATGG	ATGGAGTAGGTCTTGCTGGT	50.0	50.0	57.8	58.0	839-1136 bp	1.0	82.0
rps15-ndhF_p3	ATCCCGTGAAATTCTCGAGC	TGGAGTAGGTCTTGCTGGTT	50.0	50.0	58.1	58.3	848-1145 bp	1.0	81.5
rps15-ndhF_p4	GAGCCGAAAGATGGATGCAT	ATGGAGTAGGTCTTGCTGGT	50.0	50.0	58.4	58.0	833-1130 bp	1.0	80.6
rps15-ndhF_p5	GTTTGCTGGCTTATTTGGCA	ATGGAGTAGGTCTTGCTGGT	45.0	50.0	57.5	58.0	326-623 bp	1.0	80.4