Hotspot

clpP-psbB

LSC · 70627-71154 · 528 bp

Back to Chasmanthium

Pi 0

MarkerSeek score 60.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 528
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.9	58.0	640-660 bp	1.0	81.5
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	TATGCACCGAAAGCAATCGA	50.0	45.0	58.1	58.0	643-663 bp	1.0	81.3
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.8	58.0	600-620 bp	1.0	81.1
clpP-psbB_p4	ACCCAAGTCGCTTCTTCATC	TATAAAGCCATCGAGCCAGC	50.0	50.0	57.9	57.8	689-709 bp	1.0	80.1
clpP-psbB_p5	ACCCAAGTCGCTTCTTCATC	CCAGCCCAACCAGAAACTAG	50.0	55.0	57.9	58.2	674-694 bp	1.0	80.1