Hotspot

rbcL-psaI

LSC · 58514-59447 · 934 bp

Back to Chasmanthium

Pi 0.00321

MarkerSeek score 60.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 3
pi: 0.00321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGATCAAATTCGAGTTCGCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1045 bp	1.0	79.8
rbcL-psaI_p2	CGATCAAATTCGAGTTCGCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1051 bp	1.0	79.2
rbcL-psaI_p3	GATCAAATTCGAGTTCGCGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1044 bp	1.0	77.8
rbcL-psaI_p4	GCGATCAAATTCGAGTTCGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1046 bp	1.0	77.8
rbcL-psaI_p5	GAGTCCTGAACTAGCTGCAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.7	58.0	1083 bp	1.0	77.7