Hotspot

trnD-GUC-psbM

LSC · 17255-18310 · 1056 bp

Back to Chasmanthium

Pi 0.0019
MarkerSeek score 59.90
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1056
variable_sites
2
pi
0.0019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.5 56.7 1220-1221 bp 1.0 66.0
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG AATCATTTTGGCTGGCTGTT 50.0 40.0 58.5 56.5 1222-1223 bp 1.0 64.1
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG ATCATTTTGGCTGGCTGTTT 50.0 40.0 58.5 56.5 1221-1222 bp 1.0 64.1
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTTT 50.0 38.1 58.5 57.4 1220-1221 bp 1.0 63.9
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG TTTGGCTGGCTGTTTTTACA 50.0 40.0 58.5 56.3 1216-1217 bp 1.0 63.2