Hotspot

trnG-UCC-trnT-GGU

LSC · 14203-16015 · 1813 bp

Back to Chasmanthium

Pi 0.00112

MarkerSeek score 59.80

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1813
variable_sites: 2
pi: 0.00112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	1913-1933 bp	1.0	41.8
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	1883-1903 bp	1.0	41.7
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	GACTTCTGTCTTACCAAGGCA	38.1	47.6	55.5	57.6	1941-1961 bp	1.0	41.4
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	TGACTTCTGTCTTACCAAGGC	38.1	47.6	55.5	57.6	1942-1962 bp	1.0	41.4
trnG-UCC-trnT-GGU_p5	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	1879-1899 bp	1.0	40.9