Hotspot

trnG-GCC-trnfM-CAU

LSC · 12900-13275 · 376 bp

Back to Chasmanthium

Pi 0.0275

MarkerSeek score 65.20

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 10
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CCGTAGCAAGCGTAGTTCAA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.3	57.8	506-518 bp	1.0	79.2
trnG-GCC-trnfM-CAU_p2	CCGTAGCAAGCGTAGTTCAA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.3	58.3	521-533 bp	1.0	78.7
trnG-GCC-trnfM-CAU_p3	CCGTAGCAAGCGTAGTTCAA	CAGTTTTCGGCCCTACAGAG	50.0	55.0	58.3	58.3	551-563 bp	1.0	78.6
trnG-GCC-trnfM-CAU_p4	CCGTAGCAAGCGTAGTTCAA	CTACAGAGCGGAGTAGAGCA	50.0	55.0	58.3	58.3	539-551 bp	1.0	78.2
trnG-GCC-trnfM-CAU_p5	CCGTAGCAAGCGTAGTTCAA	TACAGAGCGGAGTAGAGCAG	50.0	55.0	58.3	58.3	538-550 bp	1.0	78.2