Hotspot

matK-trnK-UUU

LSC · 3211-3882 · 672 bp

Back to Chasmanthium

Pi 0.00744

MarkerSeek score 61.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 5
pi: 0.00744
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TTCCTGAAAGAGAAGTGGGT	TTCAATGTTTTGACAGGCGC	45.0	45.0	56.0	57.9	859-879 bp	1.0	63.2
matK-trnK-UUU_p2	ATTCCTGAAAGAGAAGTGGGT	TTCAATGTTTTGACAGGCGC	42.9	45.0	56.5	57.9	860-880 bp	1.0	59.7
matK-trnK-UUU_p3	TTCCTGAAAGAGAAGTGGGT	GTCCGGTGTAATTCCATCGA	45.0	50.0	56.0	57.4	839-859 bp	1.0	59.6
matK-trnK-UUU_p4	TGAAAGAGAAGTGGGTAAACGA	TTCAATGTTTTGACAGGCGC	40.9	45.0	57.3	57.9	855-875 bp	1.0	58.0
matK-trnK-UUU_p5	TTCCTGAAAGAGAAGTGGGT	CGTCCGGTGTAATTCCATCG	45.0	55.0	56.0	58.8	840-860 bp	1.0	57.9