Hotspot

rpl16

LSC · 79344-80826 · 1483 bp

Back to Chasechloa

Pi 0.00676

MarkerSeek score 62.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1483
variable_sites: 10
pi: 0.00676
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCTGGGG	TTTCCCAAACCAAAACCCCT	47.6	45.0	58.0	57.7	1627-1646 bp	1.0	70.1
rpl16_p2	CCAGAAAGAAAAACCTGGGGT	TTTCCCAAACCAAAACCCCT	47.6	45.0	58.3	57.7	1626-1645 bp	1.0	67.9
rpl16_p3	CCAGAAAGAAAAACCTGGGG	TTTCCCAAACCAAAACCCCT	50.0	45.0	56.5	57.7	1626-1645 bp	1.0	66.5
rpl16_p4	TCCAGAAAGAAAAACCTGGGG	TTTTCCCAAACCAAAACCCC	47.6	45.0	58.0	56.9	1628-1647 bp	1.0	63.5
rpl16_p5	TCCAGAAAGAAAAACCTGGGG	TCCCAAACCAAAACCCCTAA	47.6	45.0	58.0	56.8	1625-1644 bp	1.0	62.9