Hotspot

trnH-rpl2

IRb · 82734-82788 · 55 bp

Back to Chasechloa

Pi 0.0182
MarkerSeek score 63.00
Primer available yes
Label trnH-rpl2

Feature Metadata

label_name
trnH-rpl2
feature_type
igs
region
IRb
length_bp
55
variable_sites
1
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-rpl2_p1 CTTTAGCTGGATAAGGGGCG GTTATCCTGCGCTTGGAAGA 55.0 50.0 58.1 58.0 223 bp 1.0 79.2
trnH-rpl2_p2 CGGATGTAGCCAAGTGGATC GTTATCCTGCGCTTGGAAGA 55.0 50.0 58.1 58.0 205 bp 1.0 79.2
trnH-rpl2_p3 TGGATCAAGGCAGTGGATTG GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 191 bp 1.0 79.2
trnH-rpl2_p4 GGATCAAGGCAGTGGATTGT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 190 bp 1.0 79.1
trnH-rpl2_p5 GTGGATCAAGGCAGTGGATT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 192 bp 1.0 79.1