Hotspot

rbcL-psaI

LSC · 58015-58979 · 965 bp

Back to Chasechloa

Pi 0.0111

MarkerSeek score 56.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 10
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1336-1361 bp	1.0	84.3
rbcL-psaI_p2	GATCATATCCACGCCGGTAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1497-1522 bp	1.0	83.9
rbcL-psaI_p3	GGCTTCAGGGGGTATTCATG	AATAGGCCCGAGATTCCGTA	55.0	50.0	58.0	58.0	1959-1984 bp	1.0	83.1
rbcL-psaI_p4	GATCATATCCACGCCGGTAC	AATAGGCCCGAGATTCCGTA	55.0	50.0	58.0	58.0	2120-2145 bp	1.0	82.9
rbcL-psaI_p5	ATATCCACGCCGGTACAGTA	AATAGGCCCGAGATTCCGTA	50.0	50.0	58.0	58.0	2116-2141 bp	1.0	82.8