Hotspot

psaA-ycf3

LSC · 43508-44139 · 632 bp

Back to Chasechloa

Pi 0.00954

MarkerSeek score 64.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 6
pi: 0.00954
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	785-787 bp	1.0	80.2
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	781-783 bp	1.0	79.9
psaA-ycf3_p3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	783-785 bp	1.0	79.0
psaA-ycf3_p4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	784-786 bp	1.0	79.0
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	50.0	50.0	57.8	58.1	779-781 bp	1.0	78.7