Hotspot

psbM-petN

LSC · 18389-19177 · 789 bp

Back to Chasechloa

Pi 0.00888

MarkerSeek score 64.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 7
pi: 0.00888
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	896-918 bp	1.0	69.1
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	56.7	58.3	840-862 bp	1.0	68.1
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	56.7	58.4	839-861 bp	1.0	67.4
psbM-petN_p4	AAACAGCCAGCCAAAATGAT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	895-917 bp	1.0	67.3
psbM-petN_p5	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	894-916 bp	1.0	67.3