Hotspot

trnD-psbM

LSC · 17287-18283 · 997 bp

Back to Chasechloa

Pi 0.0156

MarkerSeek score 65.40

Primer available yes

Label trnD-psbM

Feature Metadata

label_name: trnD-psbM
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 12
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-psbM_p1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	952-1162 bp	1.0	70.7
trnD-psbM_p2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	55.0	40.0	58.7	56.7	984-1194 bp	1.0	69.3
trnD-psbM_p3	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.5	56.5	954-1164 bp	1.0	68.9
trnD-psbM_p4	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.5	56.5	953-1163 bp	1.0	68.9
trnD-psbM_p5	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.5	57.4	952-1162 bp	1.0	68.6