Hotspot

trnG-trnM

LSC · 14097-16046 · 1950 bp

Back to Chasechloa

Pi 0.0119

MarkerSeek score 59.70

Primer available yes

Label trnG-trnM

Feature Metadata

label_name: trnG-trnM
feature_type: igs
region: LSC
length_bp: 1950
variable_sites: 16
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnM_p1	ACGAATCACACTTTTACCACT	TCGAACCGATGACTTCTGTC	38.1	50.0	55.5	57.4	1500-2102 bp	1.0	51.4
trnG-trnM_p2	ACGAATCACACTTTTACCACT	CGAACCGATGACTTCTGTCT	38.1	50.0	55.5	57.4	1499-2101 bp	1.0	51.3
trnG-trnM_p3	AGAACGAATCACACTTTTACCAC	TCGAACCGATGACTTCTGTC	39.1	50.0	57.3	57.4	1503-2105 bp	1.0	50.3
trnG-trnM_p4	GAACGAATCACACTTTTACCACT	TCGAACCGATGACTTCTGTC	39.1	50.0	57.3	57.4	1502-2104 bp	1.0	50.3
trnG-trnM_p5	GAACGAATCACACTTTTACCACT	CGAACCGATGACTTCTGTCT	39.1	50.0	57.3	57.4	1501-2103 bp	1.0	50.3