Hotspot

trnS-psbD

LSC · 8377-9354 · 978 bp

Back to Chasechloa

Pi 0.0143

MarkerSeek score 53.10

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 14
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	AAGGAAAGAGCAATAGGCCG	45.0	50.0	58.0	58.0	1137-1139 bp	1.0	80.5
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	AGCGATTGGAGCAGAGAATG	45.0	50.0	58.0	58.0	1472-1474 bp	1.0	80.3
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	CAGCGATTGGAGCAGAGAAT	45.0	50.0	58.0	58.0	1473-1475 bp	1.0	80.3
trnS-psbD_p4	ATTAGCAATCCGCCGCTTTA	CGACAAAAGTCCACAGACCA	45.0	50.0	58.0	58.1	1362-1364 bp	1.0	80.3
trnS-psbD_p5	ATTAGCAATCCGCCGCTTTA	ATATCGCTGCTACGCCAAAA	45.0	45.0	58.0	58.1	1554-1556 bp	1.0	80.2