Hotspot

rps16-trnQ

LSC · 5585-7053 · 1469 bp

Back to Chasechloa

Pi 0.0098

MarkerSeek score 47.80

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1469
variable_sites: 6
pi: 0.0098
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	686-1543 bp	1.0	82.7
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	697-1554 bp	1.0	82.2
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	690-1547 bp	1.0	81.6
rps16-trnQ_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	701-1558 bp	1.0	81.1
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	692-1549 bp	1.0	79.9