Hotspot

trnK-rps16

LSC · 3941-4491 · 551 bp

Back to Chasechloa

Pi 0.0147
MarkerSeek score 52.70
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
551
variable_sites
8
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 TGAGGATCAGTCGTGGTCTT ATACGTTTCTAGGGGAGGGG 50.0 55.0 58.1 57.9 940-946 bp 1.0 80.1
trnK-rps16_p2 TGAGGATCAGTCGTGGTCTT TTCTAGGGGAGGGGTTGTTT 50.0 50.0 58.1 57.9 934-940 bp 1.0 79.8
trnK-rps16_p3 TGAGGATCAGTCGTGGTCTT TTTCTAGGGGAGGGGTTGTT 50.0 50.0 58.1 57.9 935-941 bp 1.0 79.8
trnK-rps16_p4 TGAGGATCAGTCGTGGTCTT TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.1 58.2 967-973 bp 1.0 79.0
trnK-rps16_p5 TGAGGATCAGTCGTGGTCTT TTTCTCGAGCCGTATGAGGA 50.0 50.0 58.1 58.2 971-977 bp 1.0 78.9