Hotspot

ycf1

SSC · 124635-129809 · 5175 bp

Back to Chamaesium

Pi 0.0201

MarkerSeek score 58.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5175
variable_sites: 341
pi: 0.0201
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGACAAACGGATGGGTTC	TCCCAGAATTTGTGCCACTT	50.0	45.0	58.1	57.6	499-565 bp	1.0	83.6
ycf1_p2	ACAGACAAACGGATGGGTTC	TTCCCAGAATTTGTGCCACT	50.0	45.0	58.1	57.6	500-566 bp	1.0	83.6
ycf1_p3	GACAAACGGATGGGTTCGAT	TCCCAGAATTTGTGCCACTT	50.0	45.0	58.3	57.6	496-562 bp	1.0	82.4
ycf1_p4	ACGAAATGCCTACGGTTTGA	TCCCAGAATTTGTGCCACTT	45.0	45.0	57.8	57.6	552 bp	0.555556	60.6
ycf1_p5	ACGAAATGCCTACGGTTTGA	TTCCCAGAATTTGTGCCACT	45.0	45.0	57.8	57.6	553 bp	0.555556	60.5