Hotspot

atpB-rbcL

LSC · 55060-55825 · 766 bp

Back to Chamaesium

Pi 0.029

MarkerSeek score 75.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 75
pi: 0.029
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACAGATACGCCCCCATGA	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1579-1612 bp	1.0	86.8
atpB-rbcL_p2	CGGTTCATTCATCTGACCGT	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1710-1743 bp	1.0	86.5
atpB-rbcL_p3	ATACAGATACGCCCCCATGA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2010-2041 bp	1.0	85.8
atpB-rbcL_p4	CGGTTCATTCATCTGACCGT	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2141-2172 bp	1.0	85.6
atpB-rbcL_p5	CGGTTCATTCATCTGACCGT	GGGGACGACCATACTTGTTC	50.0	55.0	58.0	58.0	1991-2024 bp	1.0	85.6