Hotspot

psaA-ycf3

LSC · 42443-43149 · 707 bp

Back to Chamaesium

Pi 0.0302

MarkerSeek score 77.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 67
pi: 0.0302
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTGACTTCTGGTTCCGGC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.7	57.8	729-779 bp	1.0	84.3
psaA-ycf3_p2	TTTTGACTTCTGGTTCCGGC	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.7	58.2	733-783 bp	1.0	84.1
psaA-ycf3_p3	TTTTGACTTCTGGTTCCGGC	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.7	58.3	728-778 bp	1.0	83.3
psaA-ycf3_p4	TTTTGACTTCTGGTTCCGGC	TCACGAGGCGTTTCGAATAA	50.0	45.0	58.7	57.6	722-772 bp	1.0	82.9
psaA-ycf3_p5	TTTGACTTCTGGTTCCGGC	TTGAAGATCACGAGGCGTTT	52.6	45.0	58.0	57.8	728-778 bp	1.0	81.7