Hotspot

trnD-GUC-trnY-GUA

LSC · 30963-31071 · 109 bp

Back to Chamaesium

Pi 0.0428

MarkerSeek score 74.30

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 15
pi: 0.0428
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	175-180 bp	1.0	82.8
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	174-179 bp	1.0	82.8
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	196-201 bp	1.0	81.0
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	195-200 bp	1.0	80.8
trnD-GUC-trnY-GUA_p5	TTGAATCTGTCGGGACTGAC	TGGCAATATGTCTACGCTGG	50.0	50.0	57.3	57.8	229-234 bp	1.0	78.7