Hotspot

psbM-trnD-GUC

LSC · 30342-30888 · 547 bp

Back to Chamaesium

Pi 0.0289

MarkerSeek score 74.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 51
pi: 0.0289
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCCCGTAATGCTTTTTCCT	TCTCTTTCAAGGAGGCAACG	45.0	50.0	58.1	57.8	1401-1577 bp	0.777778	77.5
psbM-trnD-GUC_p2	TGCCCGTAATGCTTTTTCCT	TGGCAATATGTCTACGCTGG	45.0	50.0	58.1	57.8	1243-1419 bp	0.777778	77.0
psbM-trnD-GUC_p3	TGCCCGTAATGCTTTTTCCT	GGCAATATGTCTACGCTGGT	45.0	50.0	58.1	57.8	1242-1418 bp	0.777778	77.0
psbM-trnD-GUC_p4	TGCCCGTAATGCTTTTTCCT	GTTCTTCTTGGGTCTGGGTC	45.0	55.0	58.1	57.8	1302-1478 bp	0.666667	71.8
psbM-trnD-GUC_p5	TGCCCGTAATGCTTTTTCCT	CTGCCGGATCCCTTATTTCC	45.0	55.0	58.1	58.1	1116-1179 bp	0.444444	61.2