Hotspot

atpF-atpH

LSC · 13282-13646 · 365 bp

Back to Chamaesium

Pi 0.0548

MarkerSeek score 81.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 365
variable_sites: 56
pi: 0.0548
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	57.9	58.2	358-458 bp	1.0	89.5
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	359-459 bp	1.0	88.9
atpF-atpH_p3	TGGCCCAAAGAAACGAAAGA	TGGCCTGGTTGTAGCATTAG	45.0	50.0	57.9	57.6	360-460 bp	1.0	88.2
atpF-atpH_p4	AGTGGCCCAAAGAAACGAAA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	58.2	58.2	360-460 bp	1.0	88.2
atpF-atpH_p5	GTGGCCCAAAGAAACGAAAG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	361-461 bp	1.0	87.6