Hotspot

trnG-UCC-trnR-UCU

LSC · 9995-10254 · 260 bp

Back to Chamaesium

Pi 0.0502

MarkerSeek score 74.30

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 40
pi: 0.0502
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.0	58.1	506-562 bp	1.0	91.1
trnG-UCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	GGGATCCGCAGCTCAAATTA	50.0	50.0	58.0	58.0	855-911 bp	1.0	90.6
trnG-UCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	GGGATCCGCAGCTCAAATTA	55.0	50.0	58.1	58.0	858-914 bp	1.0	90.4
trnG-UCC-trnR-UCU_p4	AGCTCGGTGGAAACAAAACT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.9	58.1	811-868 bp	1.0	90.4
trnG-UCC-trnR-UCU_p5	AGCTCGGTGGAAACAAAACT	GGGATCCGCAGCTCAAATTA	45.0	50.0	57.9	58.0	1160-1217 bp	1.0	89.7