Hotspot

trnS-GCU-trnG-UCC

LSC · 8596-9224 · 629 bp

Back to Chamaesium

Pi 0.0387

MarkerSeek score 81.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 74
pi: 0.0387
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	TACCACTAAACTATACCCGC	50.0	45.0	57.6	54.0	672-800 bp	1.0	56.3
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	TACCACTAAACTATACCCGC	55.0	45.0	58.5	54.0	667-795 bp	1.0	55.5
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	TTACCACTAAACTATACCCGC	50.0	42.9	57.6	54.9	673-801 bp	1.0	55.2
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	TTACCACTAAACTATACCCGC	55.0	42.9	58.5	54.9	668-796 bp	1.0	54.4
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	TTTACCACTAAACTATACCCGC	50.0	40.9	57.6	55.6	674-802 bp	1.0	53.5