Hotspot

trnK-UUU-rps16

LSC · 4265-5140 · 876 bp

Back to Chamaesium

Pi 0.029
MarkerSeek score 73.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
876
variable_sites
84
pi
0.029
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG TGCTGCCATTTTGGAAGGAT 50.0 45.0 58.0 58.1 1389-1647 bp 0.888889 85.6
trnK-UUU-rps16_p2 TAAGAACAGAGGCTCGTTGC GATTCAAAGTGTATCGCGCG 50.0 50.0 57.9 58.0 1936-1982 bp 0.888889 84.0
trnK-UUU-rps16_p3 TCCCTCGCTTCATCCAAATG GATTCAAAGTGTATCGCGCG 50.0 50.0 58.0 58.0 1673-1719 bp 0.777778 79.9
trnK-UUU-rps16_p4 TAAGAACAGAGGCTCGTTGC CGATTCAAAGTGTATCGCGC 50.0 50.0 57.9 58.0 1937-1983 bp 0.777778 78.2
trnK-UUU-rps16_p5 TCCCTCGCTTCATCCAAATG CGATTCAAAGTGTATCGCGC 50.0 50.0 58.0 58.0 1674-1720 bp 0.666667 74.1