Hotspot

rps16

LSC · 5081-6214 · 1134 bp

Back to Chamaerhodos

Pi 0.00235
MarkerSeek score 42.10
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1134
variable_sites
5
pi
0.00235
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AAACAGGGTTATTCCTTTGT TGTACCAACCGAACCAATGA 35.0 45.0 52.5 57.1 1250-1253 bp 1.0 40.5
rps16_p2 AACAGGGTTATTCCTTTGTT TGTACCAACCGAACCAATGA 35.0 45.0 52.5 57.1 1249-1252 bp 1.0 40.5
rps16_p3 ACAGGGTTATTCCTTTGTTT TGTACCAACCGAACCAATGA 35.0 45.0 52.5 57.1 1248-1251 bp 1.0 40.5
rps16_p4 AACAGGGTTATTCCTTTGTTT TGTACCAACCGAACCAATGA 33.3 45.0 53.5 57.1 1249-1252 bp 1.0 40.5
rps16_p5 ACAGGGTTATTCCTTTGTTTT TGTACCAACCGAACCAATGA 33.3 45.0 53.5 57.1 1248-1251 bp 1.0 40.5