Hotspot

ndhF

SSC · 110023-112263 · 2241 bp

Back to Chamaerhodos

Pi 0.000625

MarkerSeek score 35.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 3
pi: 0.000625
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	GGAATAACCGAACCAAACTGC	50.0	47.6	58.0	57.8	2965-2988 bp	1.0	70.9
ndhF_p2	CCTTCCCCTTCCCCTTTTTC	GGAATAACCGAACCAAACTGC	55.0	47.6	58.1	57.8	2934-2957 bp	1.0	70.8
ndhF_p3	AGAAGATCCTTCCCCTTCCC	GGAATAACCGAACCAAACTGC	55.0	47.6	58.1	57.8	2941-2964 bp	1.0	70.5
ndhF_p4	ATCCTTCCCCTTCCCCTTTT	GGAATAACCGAACCAAACTGC	50.0	47.6	58.2	57.8	2936-2959 bp	1.0	69.6
ndhF_p5	GCAGAAGATCCTTCCCCTTC	GGAATAACCGAACCAAACTGC	55.0	47.6	57.7	57.8	2943-2966 bp	1.0	68.6