Hotspot

psaA-ycf3

LSC · 42007-42790 · 784 bp

Back to Chamaerhodos

Pi 0.00153

MarkerSeek score 55.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 3
pi: 0.00153
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTACGGGATCCCTATCGACC	GCTATAGCGCTTACTCCTGG	55.0	55.0	58.1	57.6	896-914 bp	1.0	78.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	918-936 bp	1.0	78.1
psaA-ycf3_p3	TTACGGGATCCCTATCGACC	GGAGGCTTGGTTCGATCAAG	55.0	55.0	58.1	58.6	936-954 bp	1.0	76.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	958-976 bp	1.0	75.9
psaA-ycf3_p5	TTACGGGATCCCTATCGACC	TGGAAACAAGCTATAGCGCT	55.0	45.0	58.1	57.3	905-923 bp	1.0	75.7