Hotspot

trnS-GCU-trnG-UCC

LSC · 8357-8863 · 507 bp

Back to Chamaerhodos

Pi 0.00454
MarkerSeek score 56.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
507
variable_sites
5
pi
0.00454
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 549-580 bp 1.0 56.2
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 548-579 bp 1.0 56.2
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 586-617 bp 1.0 56.1
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 585-616 bp 1.0 56.1
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA AACCGAAAGACCCTTTAACT 50.0 40.0 58.2 54.5 640-671 bp 1.0 54.6