Hotspot

ndhE-ndhG

Genome · 121816-122031 · 216 bp

Back to Chamaenerion

Pi 0.176

MarkerSeek score 82.50

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: Genome
length_bp: 216
variable_sites: 36
pi: 0.176
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GGCACATAAGAGCCCTAACC	CAATTGCTGTTGCTCGTCAA	55.0	45.0	58.0	57.6	252-339 bp	1.0	88.5
ndhE-ndhG_p2	GGCACATAAGAGCCCTAACC	TTGCTGTTGCTCGTCAATGA	55.0	45.0	58.0	58.4	249-336 bp	1.0	88.5
ndhE-ndhG_p3	GGCACATAAGAGCCCTAACC	ATTGCTGTTGCTCGTCAATG	55.0	45.0	58.0	57.4	250-337 bp	1.0	86.8
ndhE-ndhG_p4	GGCACATAAGAGCCCTAACC	AATTGCTGTTGCTCGTCAATG	55.0	42.9	58.0	58.1	251-338 bp	1.0	83.3
ndhE-ndhG_p5	GGCACATAAGAGCCCTAACC	CAATTGCTGTTGCTCGTCAAT	55.0	42.9	58.0	58.1	252-339 bp	1.0	83.3