Hotspot

ndhD-psaC

Genome · 120760-120999 · 240 bp

Back to Chamaenerion

Pi 0.025

MarkerSeek score 65.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: Genome
length_bp: 240
variable_sites: 11
pi: 0.025
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GTTGGGTTAGTGGATCGTCC	AGATGTGAATCCGCTTGTCC	55.0	50.0	58.0	58.0	421-427 bp	1.0	82.8
ndhD-psaC_p2	GTTGGGTTAGTGGATCGTCC	ATGTGAATCCGCTTGTCCAA	55.0	45.0	58.0	57.8	419-425 bp	1.0	81.5
ndhD-psaC_p3	GTTGGGTTAGTGGATCGTCC	AAGAACCGAGGACTGTGTTG	55.0	50.0	58.0	57.8	449-455 bp	1.0	80.9
ndhD-psaC_p4	GTTGGGTTAGTGGATCGTCC	CAAGAACCGAGGACTGTGTT	55.0	50.0	58.0	57.8	450-456 bp	1.0	80.9
ndhD-psaC_p5	GTTGGGTTAGTGGATCGTCC	GATGTGAATCCGCTTGTCCA	55.0	50.0	58.0	58.3	420-426 bp	1.0	80.9