Hotspot

ndhC-trnA-UAC

Genome · 53182-54541 · 1360 bp

Back to Chamaenerion

Pi 0.00642

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnA-UAC

Feature Metadata

label_name: ndhC-trnA-UAC
feature_type: igs
region: Genome
length_bp: 1360
variable_sites: 17
pi: 0.00642
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnA-UAC_p1	GGGGCTAAAACTCCGGAAAT	AAGGCATGTTGGGTCTTTGA	50.0	45.0	57.9	57.6	1519-1558 bp	1.0	79.8
ndhC-trnA-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGGCATGTTGGGTCTTTGAA	50.0	45.0	57.9	57.6	1518-1557 bp	1.0	79.8
ndhC-trnA-UAC_p3	CTAGTCGGGGCTAAAACTCC	AAGGCATGTTGGGTCTTTGA	55.0	45.0	57.4	57.6	1525-1564 bp	1.0	76.1
ndhC-trnA-UAC_p4	CTAGTCGGGGCTAAAACTCC	AGGCATGTTGGGTCTTTGAA	55.0	45.0	57.4	57.6	1524-1563 bp	1.0	76.1
ndhC-trnA-UAC_p5	GGGGCTAAAACTCCGGAAAT	GGCATGTTGGGTCTTTGAAA	50.0	45.0	57.9	56.8	1517-1556 bp	1.0	73.3