Hotspot

trnL-trnF

Genome · 50421-50718 · 298 bp

Back to Chamaenerion

Pi 0.0561

MarkerSeek score 85.40

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: Genome
length_bp: 298
variable_sites: 25
pi: 0.0561
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	331-388 bp	1.0	85.3
trnL-trnF_p2	AATCGTGAGGGTTCAAGTCC	AATCATGTGCCAGGAACCAG	50.0	50.0	57.5	58.2	352-409 bp	1.0	84.1
trnL-trnF_p3	AATCGTGAGGGTTCAAGTCC	GAATCATGTGCCAGGAACCA	50.0	50.0	57.5	58.2	353-410 bp	1.0	84.1
trnL-trnF_p4	AATCGTGAGGGTTCAAGTCC	TGAATCATGTGCCAGGAACC	50.0	50.0	57.5	58.2	354-411 bp	1.0	84.1
trnL-trnF_p5	AATCGTGAGGGTTCAAGTCC	AGGTTTGAACTGGTGACACG	50.0	50.0	57.5	58.3	334-391 bp	1.0	82.9