Hotspot

rpoB-trnC

Genome · 27853-29128 · 1276 bp

Back to Chamaenerion

Pi 0.0377

MarkerSeek score 75.70

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: Genome
length_bp: 1276
variable_sites: 55
pi: 0.0377
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	ACATTCCCCCATTTCCATCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1257-1366 bp	1.0	82.9
rpoB-trnC_p2	GTTCTTCCGTTAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1332-1441 bp	1.0	82.8
rpoB-trnC_p3	AGTTCTTCCGTTAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1333-1442 bp	1.0	82.7
rpoB-trnC_p4	CATTCCCCCATTTCCATCCG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.7	58.1	1256-1365 bp	1.0	81.4
rpoB-trnC_p5	CTTCCGTTAAGCCCTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1329-1438 bp	1.0	81.0