Hotspot

trnS-GCU-trnG

Genome · 8881-9706 · 826 bp

Back to Chamaenerion

Pi 0.0114

MarkerSeek score 61.00

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: Genome
length_bp: 826
variable_sites: 18
pi: 0.0114
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	CAATCCGACGCTTTAGTCCA	GGGATTAAGCAAACGAATCACA	50.0	40.9	58.0	57.3	868-907 bp	1.0	64.0
trnS-GCU-trnG_p2	AATCCGACGCTTTAGTCCAC	GGGATTAAGCAAACGAATCACA	50.0	40.9	58.0	57.3	867-906 bp	1.0	64.0
trnS-GCU-trnG_p3	ACTCAACGGAAAGAGAGGGA	GGGATTAAGCAAACGAATCACA	50.0	40.9	58.0	57.3	927-966 bp	1.0	63.9
trnS-GCU-trnG_p4	CAATCCGACGCTTTAGTCCA	ACAAGGGATTAAGCAAACGA	50.0	40.0	58.0	55.3	872-911 bp	1.0	63.7
trnS-GCU-trnG_p5	AATCCGACGCTTTAGTCCAC	ACAAGGGATTAAGCAAACGA	50.0	40.0	58.0	55.3	871-910 bp	1.0	63.7