Hotspot

rps15-ycf1

SSC · 125261-125687 · 427 bp

Back to Chamaelirium

Pi 0.0445

MarkerSeek score 78.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 427
variable_sites: 19
pi: 0.0445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCGAAGTAAGCCTCTGTATC	CGGCAGTCGTTTCAGTATGT	47.6	50.0	57.6	58.3	604-605 bp	1.0	68.7
rps15-ycf1_p2	ATGCGAAGTAAGCCTCTGTA	CGGCAGTCGTTTCAGTATGT	45.0	50.0	56.4	58.3	605-606 bp	1.0	67.1
rps15-ycf1_p3	TGCGAAGTAAGCCTCTGTAT	CGGCAGTCGTTTCAGTATGT	45.0	50.0	56.4	58.3	604-605 bp	1.0	67.1
rps15-ycf1_p4	GCGAAGTAAGCCTCTGTATCT	CGGCAGTCGTTTCAGTATGT	47.6	50.0	57.3	58.3	603-604 bp	1.0	66.4
rps15-ycf1_p5	ATGCGAAGTAAGCCTCTGTATC	CGGCAGTCGTTTCAGTATGT	45.5	50.0	58.0	58.3	605-606 bp	1.0	64.1