Hotspot

rpl32-trnL-UAG

SSC · 115037-115904 · 868 bp

Back to Chamaelirium

Pi 0.0278

MarkerSeek score 73.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 868
variable_sites: 23
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGCTAAATCGGTTTCCACC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	932-971 bp	1.0	84.0
rpl32-trnL-UAG_p2	TGGCTAAATCGGTTTCCACC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	933-972 bp	1.0	83.9
rpl32-trnL-UAG_p3	TGGCTAAATCGGTTTCCACC	GTCCCTTTTAGGAGATGCCG	50.0	55.0	58.2	58.0	998-1037 bp	1.0	83.3
rpl32-trnL-UAG_p4	TGGCTAAATCGGTTTCCACC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	942-981 bp	1.0	83.0
rpl32-trnL-UAG_p5	TGGCTAAATCGGTTTCCACC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.2	57.8	940-979 bp	1.0	82.4