Hotspot

infA-rps8

LSC · 80875-80998 · 124 bp

Back to Chamaelirium

Pi 0.0403

MarkerSeek score 68.60

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 5
pi: 0.0403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GAACATGCCGTTTGGAAGTG	TCGACTGGAAAGAATTGGGG	50.0	50.0	57.9	57.5	233 bp	1.0	77.4
infA-rps8_p2	ACATGCCGTTTGGAAGTGAT	TCGACTGGAAAGAATTGGGG	45.0	50.0	58.1	57.5	231 bp	1.0	77.1
infA-rps8_p3	GAACATGCCGTTTGGAAGTG	CGACTGGAAAGAATTGGGGG	50.0	55.0	57.9	58.5	232 bp	1.0	77.0
infA-rps8_p4	ACATGCCGTTTGGAAGTGAT	CGACTGGAAAGAATTGGGGG	45.0	55.0	58.1	58.5	230 bp	1.0	76.7
infA-rps8_p5	TAAACGGACTCGGAACATGC	TCGACTGGAAAGAATTGGGG	50.0	50.0	58.3	57.5	245 bp	1.0	75.5