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psbE-petL

LSC · 65378-66578 · 1201 bp

Back to Chamaelirium

Pi 0.0183

MarkerSeek score 66.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1201
variable_sites: 22
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAACCAACCAATAGAGCCGG	50.0	50.0	57.9	58.2	1379-1383 bp	1.0	79.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGAGCCGGGGTTATAGTTGA	50.0	50.0	57.9	57.8	1367-1371 bp	1.0	79.6
psbE-petL_p3	CAACCCGCAATGAATAGGGA	ACCAATAGAGCCGGGGTTAT	50.0	50.0	57.9	58.2	1373-1377 bp	1.0	79.5
psbE-petL_p4	ACTGACGAATAACCAACCCG	AAACCAACCAATAGAGCCGG	50.0	50.0	57.9	58.2	1392-1396 bp	1.0	79.3
psbE-petL_p5	ACTGACGAATAACCAACCCG	AGAGCCGGGGTTATAGTTGA	50.0	50.0	57.9	57.8	1380-1384 bp	1.0	79.3