Hotspot

psaA-ycf3

LSC · 42039-42685 · 647 bp

Back to Chamaelirium

Pi 0.00945

MarkerSeek score 64.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 647
variable_sites: 6
pi: 0.00945
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGACGTTTTTACGGGATCCC	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.6	57.9	739-751 bp	1.0	77.8
psaA-ycf3_p2	GACGTTTTTACGGGATCCCT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.6	57.9	738-750 bp	1.0	77.8
psaA-ycf3_p3	CTGGCCCATTCGTCAAAAGA	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	756-768 bp	1.0	77.1
psaA-ycf3_p4	TCTGGCCCATTCGTCAAAAG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	757-769 bp	1.0	77.1
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	778-790 bp	1.0	77.1