Hotspot

atpH-atpI

LSC · 13632-14255 · 624 bp

Back to Chamaelirium

Pi 0.0194

MarkerSeek score 69.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 12
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	681-948 bp	1.0	80.0
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	682-949 bp	1.0	78.8
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	696-963 bp	1.0	78.5
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	CGAATCCATGGAGGGTCATC	50.0	55.0	58.3	57.8	691-958 bp	1.0	78.1
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	712-979 bp	1.0	77.2