Hotspot

atpF-atpH

LSC · 12885-13385 · 501 bp

Back to Chamaelirium

Pi 0.0343

MarkerSeek score 65.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 17
pi: 0.0343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATAGCCCAAGGAAACAAAAA	ATTTATGGACTGGTCGTGGC	35.0	50.0	53.2	58.0	593-594 bp	1.0	44.4
atpF-atpH_p2	ATAGCCCAAGGAAACAAAAA	TCGTGGCATTAGCGCTTTTA	35.0	45.0	53.2	58.3	580-581 bp	1.0	43.2
atpF-atpH_p3	ATAGCCCAAGGAAACAAAAA	CGTGGCATTAGCGCTTTTAT	35.0	45.0	53.2	57.2	579-580 bp	1.0	43.2
atpF-atpH_p4	ATAGCCCAAGGAAACAAAAA	CGTGGCATTAGCGCTTTTATT	35.0	42.9	53.2	57.9	579-580 bp	1.0	43.2
atpF-atpH_p5	ATAGCCCAAGGAAACAAAAA	GGACTGGTCGTGGCATTAG	35.0	57.9	53.2	57.9	587-588 bp	1.0	43.1