Hotspot

psbI-trnS-GCU

LSC · 7472-7622 · 151 bp

Back to Chamaelirium

Pi 0.0331

MarkerSeek score 63.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 5
pi: 0.0331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCCGGACGTGAGGAATA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.2	58.0	240 bp	1.0	79.6
psbI-trnS-GCU_p2	ATCCCGGACGTGAGGAATAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.2	58.0	239 bp	1.0	79.6
psbI-trnS-GCU_p3	AATCCCGGACGTGAGGAATA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	195 bp	1.0	78.6
psbI-trnS-GCU_p4	ATCCCGGACGTGAGGAATAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	194 bp	1.0	78.6
psbI-trnS-GCU_p5	ATCCCGGACGTGAGGAATAA	TTCGAATCCCTCTCTCTCCG	50.0	55.0	58.2	58.3	191 bp	1.0	77.8