Hotspot

psbK-psbI

LSC · 6943-7360 · 418 bp

Back to Chamaelirium

Pi 0.0219

MarkerSeek score 62.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 9
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCTGCTGCAAGTTTTCGATG	GGATTGCGTCCTGGATCATT	50.0	50.0	58.7	58.0	528-534 bp	1.0	76.9
psbK-psbI_p2	AGCTGCTGCAAGTTTTCGAT	GGATTGCGTCCTGGATCATT	45.0	50.0	58.8	58.0	529-535 bp	1.0	76.1
psbK-psbI_p3	CTTTGTTTGGCAAGCTGCTG	GGATTGCGTCCTGGATCATT	50.0	50.0	58.8	58.0	541-547 bp	1.0	75.9
psbK-psbI_p4	GCTGCTGCAAGTTTTCGATG	TATTCCTCACGTCCGGGATT	50.0	50.0	58.7	58.2	543-549 bp	1.0	75.3
psbK-psbI_p5	GCTGCTGCAAGTTTTCGATG	TTATTCCTCACGTCCGGGAT	50.0	50.0	58.7	58.2	544-550 bp	1.0	75.3