Hotspot

rpl32-trnL-UAG

SSC · 83672-84274 · 603 bp

Back to Chamaegastrodia

Pi 0.0507

MarkerSeek score 77.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 603
variable_sites: 29
pi: 0.0507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTTTTTGACTGTCCGGTGGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	776-902 bp	1.0	85.1
rpl32-trnL-UAG_p2	CTTTTTGACTGTCCGGTGGA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	769-895 bp	1.0	84.7
rpl32-trnL-UAG_p3	CTTTTTGACTGTCCGGTGGA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	774-900 bp	1.0	84.5
rpl32-trnL-UAG_p4	CTTTTTGACTGTCCGGTGGA	GCCCATAGAGAAGCCGTATG	50.0	55.0	58.0	57.9	914-2603 bp	1.0	82.3
rpl32-trnL-UAG_p5	CTTTTTGACTGTCCGGTGGA	TTTGTGGCCCATAGAGAAGC	50.0	50.0	58.0	57.9	920-2609 bp	1.0	81.9