Hotspot

trnW-CCA-trnP-UGG

LSC · 41416-41575 · 160 bp

Back to Chamaegastrodia

Pi 0.0592

MarkerSeek score 75.50

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 9
pi: 0.0592
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCGTGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	221-229 bp	1.0	84.9
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCGTGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	219-227 bp	1.0	84.9
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCGTGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	222-230 bp	1.0	84.9
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCGTGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	232-240 bp	1.0	84.7
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCGTGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	233-241 bp	1.0	84.6