Hotspot

psaI-ycf4

LSC · 39311-39620 · 310 bp

Back to Chamaegastrodia

Pi 0.118

MarkerSeek score 74.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 310
variable_sites: 36
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TCCGGCAATTACAATGGCTT	AATAGACCCGCGATCCCATA	45.0	50.0	58.2	58.1	581-660 bp	1.0	84.4
psaI-ycf4_p2	TCCGGCAATTACAATGGCTT	AATCGGTCATAACCACTGCC	45.0	50.0	58.2	58.0	641-720 bp	1.0	84.3
psaI-ycf4_p3	TTCCGGCAATTACAATGGCT	AATCGGTCATAACCACTGCC	45.0	50.0	58.2	58.0	642-721 bp	1.0	84.3
psaI-ycf4_p4	TCCGGCAATTACAATGGCTT	CGGTCATAACCACTGCCTAC	45.0	55.0	58.2	58.1	638-717 bp	1.0	84.2
psaI-ycf4_p5	TTCCGGCAATTACAATGGCT	CGGTCATAACCACTGCCTAC	45.0	55.0	58.2	58.1	639-718 bp	1.0	84.2